Pure water is multi -faceted and crucial in the liquid chromatography. The following is a specific introduction to its application:

1. Flow phase

Reduce organic waste: Pure water as a flowing phase can reduce organic waste produced by organic solvents.

Improve analysis effect: The use of pure water can help improve the accuracy and sensitivity of analysis, especially in high -efficiency liquid chromatography (HPLC).

Avoid cross -pollution: Pure water can avoid cross -pollution in the flowing phase to the greatest extent, which is essential for maintaining the purity and reliability of the experimental results.

2. Pre -sample processing

Dilute samples: During the processing process of the sample, pure water is often used for dilution samples in order to better adapt to the input requirements of the chromatography system.

Reagent preparation: Pure water is used to prepare various chromatography test agents to ensure the purity and accuracy of the reaction.

3. Instrument maintenance

Equipment cleaning: Pure water is used for cleaning chromatography equipment, removing residues, preventing pollution and blocking, and extending the service life of the equipment.

System balance: Before the chromatography analysis, use pure water to balance the color spectrum column to restore its optimal performance.

4. Analysis process

Base line stability: The use of pure water as a flow phase can maintain the stability of the baseline during the chromatography analysis process to reduce the emergence of abnormal peak values.

Accuracy of data: The extremely low impurities in pure water help improve the accuracy and accuracy of the data, especially when the trace element analysis is more critical.

5. Environmental friendship

Green Chemistry: The application of pure water is in line with the principles of green chemistry, reducing pollution to the environment and potential harm to the human body.

Cost benefits: Compared with organic solvents, pure water is usually more economical and helps reduce the operating cost of the laboratory.

6. Security

Operation safety: The use of pure water reduces the storage and treatment risk of flammable and explosive organic solvents, and improves the safety of the laboratory.

Health protection: Avoid the health risks caused by organic solvents that may cause operators, such as inhalation of harmful vapor or skin contact.

7. Technological development

Innovative application: With the development of liquid chromatography technology, the application of pure water in the new chromatography method is also continuously expanding, such as UHPLC.

Standard formulation: The quality standards of pure water are of great significance to ensure the consistency and comparability of chromatography.

With the continuous progress of purified technology, higher purification water will become possible, further promoting the development of liquid chromatography technology.