Pure water is used in many steps of the tissue or cell culture process, It is the main component of buffers and media, it is used for the dissolution of additives and drugs, and for rinsing bioreactors, plasticware and glassware. Thus, water quality may play an important role in cell culture experimental outcomes.
As an important cultural component of in vitro cell culture processes, Cell culture has high requirements for water quality, even very small impurities will affect the survival and growth of cells, and even lead to cell death.
Cell Culture Water Requirements
1.Bacteria < 0.01 cfu/mL
2. Endotoxin <0.03EU/mL
3. TOC <5 ppb
4.Resistivity ≥18 MΩ•cm(@25℃)
Innova Water Purification System Provides Ultra Pure Water for Cell Culture
In the process of cell culture, the allocation of culture medium and reagents need to be strictly purified by a professional water Purification machine， even the cell culture glassware should be rinsed with ultra-pure water at least three times after cleaning. Innova's all-in-one water purification system machine can directly produce Type I ultra-pure water from tap water. Type I ultrapure water produced by pretreatment module, RO membrane, EDI, 185 & 254nm UV lamp, ultra purification cartridge, and endotoxin terminal filter is dispensed from the dispenser arm. Innova's all-in-one water purification system machine provides high-quality, stable ultra-pure water for cell culture, which fully meets the requirements of cell culture water.
Technical Specifications of Innova Ultra Pure Water Purification System
Here is some important technical specifications of type I ultra-pure water produced by Innova Nova EU and Performa EU water purification system:
1. Bacteria < 0.01 cfu/mL
2.Endotoxin< 0.001EU/mL
3.TOC< 2 ppb
4.Resistivity ≥18.2 MΩ.cm(@25℃)
Powerful Configuration of Innova Water Purification System to Support Cell Culture
Endotoxin (Pyrogens)
Retains 99% of substances (molecular weight /Daltons > 13000Da), effectively removes Pyrogen (endotoxins), nucleases, etc. in water.
Produces sterile, DNase-free, RNase-free ultrapure water suitable for DNA sequencing, PCR, etc. in culture, biochemical analysis and molecular biology.
Pre-treatment Module
The pretreatment module is equipped with ultrafiltration membrane. The pretreatment module can effectively remove TOC, particles, bacteria and so on. And equipped with automatic cleaning program to ensure the quality of the produced water.
Two-stage RO Module
The two-stage RO module is equipped with RO membrane, which can remove ion, TOC, particles, bacteria. Stable quality of purified water, 100% recycled in the second stage, save water and maintain costs.
EDI Module
EDI module can effectively remove ion, has the regeneration function, long service life and does not need to be replaced. EDI and RO module has automatic wake-up program to ensure cleaning.
Dual Wavelength UV Lamp
185 & 254nm UV lamp has the function of sterilizing and oxidizing organic matter.
Purification Cartridges
Pre-guard cartridge A
Filled with high quality coconut shell activated carbon removes residual chlorine, macromolecular organics, etc. effectiyely. Appropriate amount of silicon phosphorus crystal can effectively reduce the hardness of water.
Pre-guard cartridge B
Filled with high quality coconut shell activated carbon, effectiyely absorbs residual chlorine, macromolecular organics, colloids and heavy metal ions.
Ultra purification cartridge B
Filled with electronic grade ion exchange resin, which can control the ions in water at ultra trace level.
Other Innova Solutions Could Support for Cell Culture
Innova Cell Glass Bioreactor
The cell culture glass bioreactors are designed to pump the outcomes of laboratory research and such experiments with powerful performance.
Innova INOCD-2 Carbon Dioxide Incubator
A carbon dioxide incubator is a kind of scientific experimental equipment that artificially simulates the suitable environment for cell growth in vitro, and it is one of the conventional instruments commonly used in laboratories.
Innova ZWYC-290A Shaking Incubator
ZWYC-290A Ultimate-cell Stackable Shaking Incubator can be stacked up to 2-3 units high to offer multiplied incubation capacity on a single unit footprint, which could offer a great support for cell culture through temperature control, active humidity control, concentration controls, ect.
Innova offers a large variety of lab equipment and has been committed to delivering the highest quality lab equipment to medical device companies and laboratories worldwide. To learn more, visit our website https://www.innovabiomed.com, or contact us via info@innobiomed.com.
