Pure water plays a crucial role in the preparation of recombinant protein, and its application is mainly reflected in the following aspects:

The role of purified water in recombinant protein preparations

1.Cell culture medium preparation: Pure water is used to prepare impurity-free cell culture medium to ensure a pure environment for recombinant proteins during expression.

2.Sample Preparation and Washing: During the preparation of recombinant proteins, pure water is used to wash the cells and resuspend the bacteria to remove impurities and ensure the accuracy of subsequent steps.

3.Buffer Preparation: Pure water is used to prepare a variety of buffers that play a key role in steps such as induction of expression, denaturation, and replication of recombinant proteins.

4.Protein purification: Pure water is a key reagent in the protein purification process. In purification steps such as affinity chromatography and ion exchange chromatography, pure water is used as an eluent to elute recombinant proteins bound to the immobilizer in order to obtain high purity recombinant proteins.

5. Final Product Handling: After recombinant protein purification is complete, pure water is used to dilute and store the final product to ensure product purity and stability.

Effect of pure water quality on recombinant protein preparation

Impurity content: The presence of impurities in pure water affects the purity and activity of recombinant proteins, so the use of high purity water is the key to ensuring the amount of recombinant proteins.

pH and conductivity: The pH and conductivity of the purified water need to be tightly controlled to accommodate different purification steps and recombinant protein properties.

The significance of pure water in recombinant protein experimental process

Ensure accurate experimental results: Impurities in pure water, such as bacteria, pyrogens, RNA enzymes, DNA enzymes, etc., can negatively affect the results of biopharmaceuticals. Therefore, the use of pure water is essential to ensure the accuracy of experimental results.

Meet the requirements of GMP regulations: In the biopharmaceutical process, it is necessary to use pure water that complies with GMP regulations to ensure the quality of the product and the reliability of the experimental results.

Pure water is indispensable in the process of recombinant protein preparation, from sample preparation to protein purification, each step requires the use of high-purity water, the quality of which directly affects the purity and activity of recombinant protein. Therefore, in the preparation process, it is necessary to choose the appropriate method of pure water preparation and strictly control the quality of pure water, which not only ensures the accuracy of the experimental results, but also meets the requirements of GMP regulations in the process of biopharmaceuticals.