The application of pure water in the sequencing industry is multi -faceted, covering all aspects of sample preparation to instrument maintenance. The following is a specific introduction to its application:
1 sample preparation
DNA extract: DNA needs to be extracted from biological samples during gene sequencing. The use of pure water can ensure that impurities are not introduced during the extraction process, and the purity and integrity of DNA can be guaranteed.
Sample dilution: When performing PCR amplification or other molecular biology experiments, pure water is often used to dilute DNA samples to adapt to different experimental conditions.
2 sequencing reaction
Preparation of the reaction system: In sequencing reactions, pure water is the basic solvents that formulate the reaction system for dissolving various reagents, such as primers and nucleotides.
Enzyme activity is maintained: The ions and other impurities in pure water are extremely low, which helps maintain the activity of key enzymes such as DNA polymerase, thereby ensuring the accuracy and efficiency of sequencing reactions.
3 Equipment cleaning and maintenance
Instrument cleaning: sequencing instruments need to be cleaned and maintained regularly to remove residual chemical and biological pollution. The use of pure water can reduce the corrosion and pollution of the instrument and extend its service life.
Capillary maintenance: In the capillary tube of the sequencer, pure water is used to rinse and fill to prevent blockage and pollution and ensure accurate data transmission.
4 Analysis process
Base line stability: The solvent using pure water as sequencing reactions can maintain the stability of the baseline in the sequencing process and reduce the emergence of abnormal peak values.
Accuracy of data: The extremely low impurities in pure water help improve the accuracy and accuracy of the data, especially when the trace element analysis is more critical.
5 Technical innovation and development
New technology application: With the advancement of sequencing technology, such as the development of emerging technologies such as single -molecule sequencing and nano -hole sequencing, the demand for pure water is also changing, and higher water quality standards are required.
Future trend: It is expected that the use of ultra -pure water in the laboratory in the future will tend to modularize and miniaturize to meet the rapidly changing scientific research needs and cost benefits.
6. Regulations to obey
GMP requirements: Good production specifications (GMP) require that the water quality used in sequencing experiments must meet certain standards, and the use of pure water is the key to meeting this requirement.
Certification requirements: The sequencing laboratory may need to pass a specific certification to prove that the pure water they use meets international standards.
In general, the application of pure water in the sequencing industry is not only reflected in its basic function as the basic reagent, but also involves many aspects such as sample preparation, equipment maintenance, environmental control, analysis process, and technological innovation. These applications show the importance of improving the efficiency, accuracy and safety of pure water, and also pointed out its extensive prospects of scientific research and clinical diagnosis.
