Confocal Microscope：Simple, Efficient and Highly Integrated:

LC950 laser confocal microscope is a high-end product in INNOVA Optics microscope series.  It is designed  as an essential microscopy tool for laboratory scientific research, providing powerful and stable imaging capabilities and highly integrated motorization capabilities.

Obtaining high signal-noise ratio images based on high-sensitivity photomultiplier light (PMT) and stable laser light source. At the same time, the system adopts high-speed scanning galvanometer to realize real-time scanning up to 4096x4096 Resolution, the use of large numerical aperture objective  (100 times, N.A = 1.45) ensures high-quality imaging resolution.

LC950's Biological Applications：

Observation and measurement of organelles:

Specific labelling of lysosomes, mitochondria, endoplasmic reticulum, nucleus and other components of intracellular specific structures, observation of the distribution, movement and changes of the above organelles, involving studies on cell migration, targeting and localisation delivery of nano-polymer material carriers, apoptosis and so on.

Cytoskeleton research:

Labelling of specific proteins such as actin Actin, microtubulin Tubulin and other proteins in cells enables researchers to track and study cytoskeleton dynamics.

Cell Activity, Proliferation and Cell Function:

Labelling of intracellular metabolites, nucleic acid analogues, proteases and other specific molecules to study cell growth. As shown on the right, a rapid measurement of neuronal proliferation.

Apoptosis and Autophagy:

Used to study the process of apoptosis and autophagy activity by localising and tracking specific molecules such as caspases, lysosomal membranes, lysosomal inclusions, etc. in cells.

Neurobiology:

Obtain clear images of neuronal fine structure and morphological changes in tissue samples of a given thickness. Applications in brain and neuroscience Confocal microscopy is used to observe the internal structure of neural axons, the course of neural axons in brainstem tissue, and the three-dimensional structure of neural axons by layered scanning, and to observe subtle lesions in neural tissues that are not detected by ordinary light microscopy.

Developmental Biology:

Model organisms, e.g. Drosophila, nematode and zebrafish embryos, to obtain 3D structural details and dynamic changes.

Real-time quantitative detection of intracellular ionic changes:

Using fluorescent probes and dyes, intracellular calcium ions, magnesium ions, pH and membrane potential are detected and observed.

As shown in the figure on the right, NG-108 cell membrane potential is analysed.

Oxidative Stress Detection：

Detection of reactive oxygen species (ROS) substances in cells to study atherosclerosis, cancer, ischaemia-reperfusion injury and neurodegenerative diseases. Left, Detection of ROS in U2-OS cells (green).

Live cell imaging:

3D real-time imaging of mouse embryos (top)

Methylated DNA detection. (bottom)

Phagocytosis, endocytosis, receptor internalisation:

Labelling of particles or protein conjugates ingested by cells to monitor multiple processes of cellular uptake and internalisation.

Hela cells, nucleus (purple-red), early endosomes (green), peroxisomes (red), lysosomes (blue)