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Liquid Nitrogen Tanks -- ‘Time Refrigerators’ for Cells and Tissues

Liquid Nitrogen Tanks -- ‘Time Refrigerators’ for Cells and Tissues

March 06, 2025

Liquid Nitrogen Tank, full name Liquid Nitrogen Storage Vessel, is a cryogenic equipment specialized in storing liquid nitrogen (-196℃). Its core function is to preserve biological samples (such as cells, embryos, tissues, etc.) or special materials for a long period of time in an extremely low-temperature environment, preventing them from being inactivated or deteriorated due to temperature changes. Simply put, it is the “time stopper” of the scientific community.

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Liquid Nitrogen Tanks are vital as cryogenic storage and transportation equipment in fields such as biomedical and life sciences. It originated in the early twentieth century when liquid nitrogen was stored in specially designed double-walled containers for use in laboratories and specific industries. With the advancement of technology and increased demand for cryogenic applications, liquid nitrogen tanks are widely used in various industries. During the development of the liquid nitrogen tank industry, product design and materials are constantly upgraded and improved, and the technological development trend is characterized by more comfortable, convenient and intelligent.

Liquid nitrogen tanks are mostly double-layer vacuum structures, with the outer layer silver-plated to reflect heat and the inner layer filled with adiabatic material to minimize the evaporation of liquid nitrogen and ensure the stability of the low-temperature environment. The boiling point of liquid nitrogen is -196℃, and the temperature inside the tank is stably maintained between -150℃ and -196℃, which can almost completely inhibit the biochemical reaction of the samples and realize long-term preservation.

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Specimens to be loaded into liquid nitrogen tanks

1. Cell lines and cell culture

Application: It is often necessary to preserve various cell lines in the laboratory for subsequent research.

Features: By suspending cells in cryoprotectant and freezing them quickly, damage to cell membranes caused by ice crystal formation can be avoided. Liquid nitrogen tanks provide ideal storage conditions to ensure high cell viability even after resuscitation.

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2. Biological tissue

Application: Including human or animal tissue samples for research in pathology, genetics and other fields.

Features: Liquid nitrogen tanks can help maintain the stability of tissue structure and biomolecules (e.g. DNA, RNA) and prevent degradation. This is important for long-term storage as well as possible future research and analysis.

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Although liquid nitrogen tanks are obscure, they are the indispensable “heroes behind the scenes” of modern life sciences. From basic research to clinical treatment, it guards the hope of life with extremely cold temperature. Whether it is gas-phase liquid nitrogen tank or liquid-phase liquid nitrogen tank, as long as through the reasonable use and maintenance, can give full play to its advantages, for scientific research and medical treatment and other fields to provide effective support.






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